Photostimulation in Neurocells by UV Laser light (355 nm)
Uncaging experiments in neurocells require a UV laser to release ions or molecules. By this, biological reactions are triggered for basic research.
Biological cells can easily be destroyed by excessive UV average power or pulse peak power. Too low repetition rate and pulse energy, however, result in little or no biological effect.
The DPSS UV laser model 3505-100 provides sufficient power (500 mW) at recommended pulse energy and high pulse repetition rate (100 kHz). Cells are triggered and release molecules such as glutamat, but are not destroyed.
The 3505 DPSS laser is the most efficient, high power, quasi-CW DPSS UV laser commercially available. Using only a single 2OW diode bar, the Series 3500 has demonstrated powers in excess of 3.0 Watts at 355 nm. This high efficiency conversion means that the laser can be operated at the normal specification, with minimum stress to the optical components and with a reduced diode current to extend the operating life.
Minimizing the diode power required to generate the 355nm third harmonic also minimizes the thermal effects in the gain medium (Nd:YV04), which results in the lowest possible distortion to the beam. Both the second and third harmonics are generated intra-cavity, thus reducing the need for tight focusing into the non-linear crystals. The result is a stable, high power UV laser with an M² specification of < 1.2, a near perfect TEMoo mode.
Samples of DNA are applied to a glass slide in a series of microscopic wells. An enzyme is used to produce complimentary DNA (cDNA) which is labeled with a fluorescent dye. The cDNA is then washed over the glass slide. Wherever DNA and cDNA match up, a bond is created.
By using the DPSS Series 3500 UV Laser, researchers can fluoresce the bonded wells and generate an illuminated array of hybridizes bonds without damaging the samples. The original DNA in each well is known, therefore the illuminated array can help researchers determine the origin or functionality of the unknown enzyme. Since the fluorescence is of a longer wavelength than the native illuminating laser, the signal to noise ratio can be extremely high, thereby increasing the sensitivity of the measurement.
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